What's New and Topics (What's New?)
2024/06/26 S. pombe strains (FY48896-FY48916) and plasmids (FYP6291-FYP6298) used in Sakai et al. (2024) deposited by Dr. Y. Goto (Kyoto University, Japan) are now available on request.
2024/06/21 S. cerevisiae nucleocytoplasmic transport mutants (BY29216-BY29260) used in Belanger et al. (1994), Booth et al. (1999), Belanger et al. (2004), Belanger et al. (2005), Harper et al. (2008), Brown et al. (2021) and Belanger et al. (2022) were deposited by Dr. K. Belanger (Colgate University, USA) and are now available on request.
2024/06/07 S. cerevisiae TORC1-related mutants (BY29597-BY29628) and plasmids (BYP10403-BYP10419) used in Kamada et al. (1995),Kamada et al. (2023) and Kamada et al. (2024) were deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan) and are now available on request.
2024/06/05 S. cerevisiae plasmids expressing a variety of genes such as for glycolytic enzymes and fluorescent proteins ( BYP9968 , BYP10206-10207, BYP10231-10386 , BYP10400-10402, BYP10420-10497) ( Moriya et al. (2012) , Makanae et al. (2013) , Kintaka et al. (2016) , Eguchi et al. (2018), Abe-Kanoh et al. (2019), Namba et al. (2022)) were deposited by Dr. H. Moriya (Okayama University, Japan) and are now available on request.
2024/05/15 S. pombe TORC1 related strains (FY49390-FY49547, Chia et al. (2017)) and (FY49548-FY49603, Morozumi et al. (2023)) deposited by Dr. K. Shiozaki and Dr. Y. Morozumi (Nara Institute of Science and Technology, Japan) are now available on request.
2024/04/12 S. pombe strains (FY48878-FY48895) and plasmids (FYP6288-FYP6290) for manipulation and visualization of PKA activity used in Sakai, K., Aoki, K., Goto, Y. (2024) deposited by Dr. Y. Goto (National Institute for Basic Biology, Japan) are now available on request.
2024/04/09 S. pombe 460 strains [FY48923-FY49382 (Ono et al. (2003), Okita et al. (2019), Nitani et al. (2006), Nitani et al. (2008), Nakamura et al. (2008), Maki et al. (2011), Blaikley et al. (2014), Onaka et al. (2016), Zafar et al. (2017), Nakagawa, T., Okita, A. K. (2019), Onaka et al. (2020), Su et al. (2021), Mongia et al. (2023))] deposited by Dr. T. Nakagawa (Osaka University, Japan) are now available on request.
2024/04/03 S. pombe plasmids and strains related to Bqt4 and cdc48 (FYP6271-FYP6281, FY48837-FY48875) used in Toan Khanh Le et al. (2023) deposited by Dr. Y. Hiraoka (Osaka University, Japan) are now available on request.
2024/03/29 S. pombe Pil1 co-tethering assay plasmids deposited by Dr. Li-Lin Du (NIBS, China, unpublished) are now available on request (FYP6282-6285, FYP6299-6308). CLICK HERE for more information about these plasmids.
2024/03/28 S. pombe wee1 related strains (FY49383-49389) used in Masuda et al. (2011) deposited by Dr. T. Toda (Hiroshima University, Japan) are now available on request.
2024/03/13 S. pombe phospholipid-related strains (FY48917-FY48922) used in Willet et al. (2023) deposited by Dr. K. Gould (Vanderbilt University, USA) are now available on request.
2024/03/08 S. pombe plasmids (FYP6309-FYP6311) used in Namba et al. (2022) deposited by Dr. H. Moriya (Okayama University, Japan) are now available on request.
2024/03/04 S. cerevisiae strains ( BY29595-29596) and plasmids (BYP10387-10399) for affinity-linker based super-sensitive auxin-inducible degron (AlissAID) system used for conditional protein-knockdown ( Ogawa et al. (2023) ) were deposited by Dr. K. Nishimura, Y. Ogawa and T. P. Ueda (Nagoya University, Japan) and are now available on request.
2024/02/28 S. cerevisiae mutants ( BY29304-29366) used in the papers for analyzing cellular response against high hydrostatic pressure and the eisosomes in the plasma membrane ( Abe et al. (2003) , Suzuki et al. (2013) , Uemura et al. (2020) , Ishii et al. (2022) , Mochizuki et al. (2023) ) were deposited by Dr. F. Abe (Aoyama Gakuin University, Japan) and are now available on request.
2024/02/21 S. cerevisiae mutants ( BY29396-29398, BY29402-29594) for studying epigenetic formation and switching of heterochromatin boundary ( Donze et al. (1999) , Oki et al. (2004) , Hatanaka et al. (2011) , Kamata et al. (2013) , Mano et al. (2013) , Kamata et al. (2014) , Mitsumori et al. (2016) , Kamata et al. (2016) , Mitsumori et al. (2016) ), and S. pombe and S. cerevisiae mutants ( FY39986-39991, BY29367-29395, BY29399-29401) used in the study of Ran-GTPase ( Oki et al. (1998) , Oki et al. (1998) , Tatebayashi et al. (2001) , Oki et al. (2007) ) were deposited by Dr. M. Oki (Fukui University, Japan) and are now available on request.
2024/02/15 S. cerevisiae two-hybrid vectors (BYP10228-10230) for isolating interaction-null/impaird mutants used in Satake et al. (2023) were deposited by Dr. S. Tanaka (Kochi University of Technology, Japan) and are now available on request.
2024/02/13 S. pombe Hikeshi-related strains (FY48876-48877, unpublished) deposited by Dr. N. Imamoto (RIKEN, Japan) are now available on request.
2024/02/13 S. pombe CRISPR/Cas9 system-related plasmids (FYP6286-6287) used in Saito et al. (2023) deposited by Dr. H. Nakaoka (Kyoto University, Japan) are now available on request.
2024/01/17 S. pombe strains (FY48340-FY48445) and plasmids (FYP6151-FYP6241) for FRET biosensor system for measuring CDK activity used in Sugiyama, H. et al. (2024) deposited by Dr. Y. Goto (National Institute for Basic Biology, Japan) are now available on request.
2023/12/21 S. cerevisiae double disruption strain for TRK1 and TRK2 encoding potassium transporters (BY29296) used in ( Wright et al. (1997) ) was deposited by Dr. N. Uozumi (Tohoku University, Japan) and is now available on request.
2023/11/27 S. pombe strains (FY48446-FY48464) and plasmids (FYP6247-FYP6249) for the SLIPT system used in Nakamura, A. et al. (2023) deposited by Dr. Y. Goto (National Institute for Basic Biology, Japan) are now available on request.
2023/11/15 S. pombe casein kinase-related strains (FY48472-FY48474) used in Koyano et al., Genes to Cells, 20, 1046–1058 (2015) and Koyano et al., Molecular and Cellular Biology, 35, 1533–1542 (2015) deposited by Dr. T. Koyano (Shigei Medical Research Institute, Japan) are now available on request.
2023/10/25 Versatile plasmid vectors capable of gene introduction into S. cerevisiae via Agrobacterium-mediated yeast transformation (AMT) (BYP9798-9804, BYP10211-10214) reported by Kiyokawa et al. (2020) and Kiyokawa et al. (2023) have been deposited by Dr. K. Suzuki and Dr. K. Kiyokawa (Hiroshima University, Japan) and are now available on request.
2023/10/13 S. pombe tetracycline-inducible expression system plasmids deposited by Dr. Li-Lin Du (NIBS, China, unpublished) are now available on request (FYP6242-FYP6246). CLICK HERE for more information about these plasmids.
2023/08/04 S. pombe strains [FY41328-FY41686 (Chujo et al. (2012), Chujo, M. (2012), Irie et al. (2019), Mikawa et al. (2010), Miyoshi et al. (2009), Miyoshi et al. (2002), Nakaoka, H., Wakamoto, Y., (2017), Takikawa et al. (2017), Tarumoto et al. (2013), Tashiro et al. (2013), Yamamoto et al. (2021), Yamazaki et al. (2012)] deposited by Dr. F. Ishikawa and Dr. H. Nakaoka (Kyoto University, Japan) are now available on request.
2023/07/28 S. cerevisiae plasmids harboring meiosis-inducing genes IME1and IME2 ( BYP10208-10210) ( Matsuura et al. (1990) , Yukawa et al. (1999) ) have been deposited by Dr. M. Yukawa (Hiroshima University, Japan) and are now available on request.
2023/07/10 S. pombe CRISPR interference-ralated plasmids (FYP6145-FYP6150) used in Ishikawa et al. (2023) deposited by Dr. S. Saitoh and Dr. K. Ishikawa (Kurume University, Japan) are now available on request.
2023/06/26 S. pombe strains [FY44261-FY44332, FY44609, FY45771-FY45776, FY46115-FY47421, FY47523-FY47593 (Ruan et al. 2014, 2015), (Yang et al. 2015, 2017, 2020, 2023), (Tange et al. (2016)), (Hirano et al. (2020)), and (Martin Caballero et al. (2022))] deposited by Dr. Y. Hiraoka (Osaka University, Japan) are now available on request.
2023/06/26 S. pombe chromatin-related strains [(FY44429, FY44983, FY46031-FY46104, Matsuda et al. (2015)), and (FY46105-FY46107, Yadav et al. (2021))] deposited by Dr. A. Matsuda (NICT, Japan) are now available on request.
2023/06/22 S. pombe heterochromatin-related strains (FY47735-FY47741) used in Maki et al. (2023) deposited by Dr. H. Iwasaki (Tokyo Institute of Technology, Japan) are now available on request.
2023/06/14 S. pombe bmc1∆ strain and thc1∆ strain (FY47742-FY47743) used in Porat et al. (2022 , 2023) deposited by Dr. M. Bayfield (York University, Canada) are now available on request.
2023/06/14 S. pombe vectors and strains set for chromosome integration (FYP5995-FYP5996, FY47712-FY47734) used in Matsuyama et al. (2023) deposited by Dr. A. Matsuyama (RIKEN, Japan) are now available on request.
2023/06/08 GFP overexpression plasmids pTOW40836-PYK1pro-GFP and pTOW40836-PYK1-500pro-GFP for S. cerevisiae ((BY9897, 9898) ( Kintaka et al. (2016) and Moriya et al. (2022) ) have been deposited by Dr. H. Moriya (Okayama University, Japan) and are now available on request.
2023/05/09 S. cerevisiae BY4741 Δadh1 strain (BY29295) ( Utsumi et al. (2023) ) that is not included in the commercially available yeast KO strain collection has been deposited by Dr. N. Miura (Osaka Metropolitan University, Japan) and is now available on request.
2023/05/08 S. pombe chromosome-related strains deposited by Dr. Y. Chikashige (NICT, Japan) ((FY41101-41248, FY41327, Chikashige et al., 2004, 2006, 2007, 2009, 2014, 2017) (FY41249-41301, Yamamoto et al. (2019)) are now available on request.
2023/4/17 S. cerevisiae YEp plasmids (BYP9966-9967) for the expression of mitochondria-targeted GFP, that was used in Okamoto et al. (2016) and Ishikawa et al. (2022) , have been deposited by Dr. I. Miyakawa (Yamaguchi University, Japan) and Dr. S. Izawa (Kyoto Institute of Technology, Japan), and are now available on request.
2023/4/12 S. cerevisiae 25 YIp plasmids (BYP9941-9965) for the expression of GFP-, mRFP- or FLAG-tagged Rpn1, Rpn7, Pre6, Hsp60, Hsp78, Ssc1, Ilv2, Kgd1, Aco1, Fes1, Sis1, Ssa1, Ssa3 and Lsg1, that were used in Nguyet et al. (2022) , Ishikawa et al. (2022) and Yoshida et al. (2021) , have been deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) and are now available on request.
2023/03/22 S. pombe tubulin-related plasmids ( FYP5207-FYP5208) used in Murase et al., (2022) deposited by Dr. Sato (Waseda University, Japan) are now available on request.
2023/03/15 S. pombe CRISPR/Cas9 system-related plasmids ( FYP5203-FYP5206) used in Saito et al., (2023) deposited by Dr. Yamazaki (Niigata University of Pharmacy and Applied Life Sciences, Japan) are now available on request.
2023/03/02 S. pombe tubulin-related strains( FY41097-FY41100) used in Tang et al., (2022) or Okada et al., (2014) deposited by Dr. Toda (Hiroshima university, Japan) are now available on request.
2023/02/07 S. pombe nuclear envelope protein-related strains(FY41063-FY41096) used in Asakawa et al., (2022) deposited by Dr. Asakawa (Osaka university, Japan) are now available on request.
2023/01/26 Plasmid vectors for S. pombe with a flexible multiple cloning site and modular epitope tags (FYP5183-FYP5202) used in Sajeevan et al. (2022) deposited by Dr. T. Toda (Hiroshima University, Japan) are now available on request.
2023/1/24 S. cerevisiae reporter plasmids (BYP9939-9940) harboring the UPRE-CYC1-lacZ gene used in Kawahara et al. (1997) have been deposited by Dr. K. Mori (Kyoto University) and are now available on request.
2022/11/25 S. cerevisiae beta-est AID plasmid (BYP9938) used in Mendoza-Ochoa et al. (2019) has been deposited by Dr. Jean D. Beggs (University of Edinburgh, UK) and is now available on request.
2022/10/27 S. pombe kinesin-related strains (FY41058-FY41052) and plasmids (FYP5177) used in Tang NH and Toda T,(2015) or Yukawa et al., (2018) have been deposited by Dr. T. Toda (Hiroshima University, Japan) are now available on request.
2022/10/11 S. pombe chromosome-related strains deposited by Dr. M. Yanagida (OIST, Japan) (FY41039-FY41049(Nakazawa et al., (2019))), (FY41050-FY41057(Xu X et al., (2019))) are now available on request.
2022/10/11 S. pombe centromere-related plasmids(FYP5179-FYP5182(Hirai et al.,(2022)))have been deposited by Dr. M. Sato (Waseda University) are now available on request.
2022/10/06 S. pombe the subunit of RNA polymerase Rpa2-related strains(FY41037-FY41038(Ishida et al.,(2022)))have been deposited by Dr. K. Kawakami (Kwansei Gakuin University) are now available on request.
2022/9/30 S. pombe TOR complex-related strains(FY40925-FY40966(Morozumi Y et al.,(2021)))have been deposited by Dr. Y. Morozumi (Nara Institute of Science and Technology) are now available on request.
2022/8/26 S. pombe chromosome-related strains(FY40760-40872(Sakuno et al., (2022)), FY40873-40924)have been deposited by Dr. Y. Hiraoka (Osaka University, Japan) are now available on request.
2022/8/8 S. cerevisiae QUEEN-2m strains (BY29261-29289) used in "High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast" (Takaine et al. 2022) have been deposited by Dr. M. Takaine (Gunma University, Japan) and are now available on request.
2022/7/11 Marine-derived yeasts (FY40737-40752) used in Goshima, (2022) have been deposited by Dr. G. Goshima (Nagoya University, Japan) are now available on request.
2022/7/1 S. pombe polo kinase- and tubulin- related strains (FY40474,40475,40477-40532) and plasmids(FYP5020-5029) used in Kim j, Goshima, (2022) and S. pombe strains(FY40533-40736) used in Takeda et al., (2019) have been deposited by Dr. G. Goshima (Nagoya University, Japan) are now available on request.
2022/6/17 S. pombe chromosome-related strains deposited by Dr. M. Yanagida (OIST, Japan) (FY40298-40473)(Xu X et al., (2015, 2018a, 2018b, 2019a, 2019b, 2022), Xu X, Yanagida, (2019))are now available on request.
2022/5/31 S. cerevisiae strains (BY29156-29212) including spf1 disruptants and strains with SMK1 derivatives in plasmids (BYP9823-9896) used in the study for SMKT (salt-mediated killer toxin) produced by the halotolerant killer yeast Millerozyma (Former Pichia) farinose KK1 have been deposited by Dr. C. Suzuki (Nihon University, Japan) and are now available on request.
2022/2/3 S. cerevisiae and O. polymorpha strains (BY10700-10855) used in Benjaphokee et al. (2012a) , Benjaphokee et al. (2012b) , etc. and plasmids (BYP4179-4309) used in Reid RJ et al. (2008) , Prasitchoke et al. (2007a) , Prasitchoke et al. (2007b) , Shahsavarani et al. (2012) , etc. have been deposited by Dr. Y. Kaneko (Osaka University, Japan) and are now available on request.
2022/1/6 S. cerevisiae mutants (BY29106-29155) used in study for DNA replication in which replication factor Sld3 and histone acetyl transferase Esa1 are involved ( Tanaka (2021) ) have been deposited by Dr. S. Tanaka (Kochi University of Technology, Japan) and are now available on request.
2022/1/6 S. cerevisiae strains (BY29103-29105) and plasmids (BYP9818-9822) for improved auxin-induced degron system (iAID) used in ( Tanaka (2020) ) have been deposited by Dr. S. Tanaka (Kochi University of Technology, Japan) and are now available on request.
2021/12/15 S. pombe strains (FY39971-39977) for Rapamycin target protein Tor deposited by Dr. T. Toda (Hiroshima University, Japan, Uritani et al. (2006))are now available on request.
2021/12/15 S. pombe strains (FY39978-39985) for RNA-binding protein Dri1 deposited by Dr. M. Yukawa (Hiroshima University, Japan, Yukawa et al. (2021)) are now available on request.
2021/9/24 S. pombe strains (FY39933-39966) and plasmids (FYP4966-5019) for iRFP and phytochrome-based optogenetics deposited by Dr. Y. Goto (National Institute for Basic Biology, Japan, Sakai et al. (2021)) are now available on request.
2021/9/15 S. pombe auxin-inducible degron system plasmids (FYP4964-4965) and strains (FY39929-39932) deposited by Dr. T. Sugiyama (Shanghai Tech University, China, Song et al. (2021)) are now available on request.
2021/9/13 A series of simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vectors for S. cerevisiae genome editing (BYP9733-9770) (BYP9805-9817) (Okada et al., (2021) ) have been deposited by Dr. S. Okada (Kyusyu University, Japan) and are now available on request. A protocol for S. cerevisiae genome editing with these plasmids was also provided by Dr. S. Okada and posted on the top page.
2020/8/27 S. pombe auxin-inducible degron system strains (FY39919-39928) and plasmids (FYP4960-4963) deposited by Dr. Li-Lin Du (NIBS, China, Zhang et al. (2021)) are now available on request.
2021/7/5 A series of simple to use CRISPR/Cas vectors using SpCas9, SaCas9 and AsCas12a with distinct PAMs for genome editing (BYP9733-9770) used in Okada et al., (2021) have been deposited by Dr. S. Okada (Kyusyu University, Japan) and are now available on request.
2021/6/18 A series of auxin-inducible degron version 2 (AID2) plasmids for S. cerevisiae (BYP9794-9797) used in Yesbolatova et al., (2020) have been deposited by Dr. M. Kanemaki (National Institute of Genetics, Japan) and are now available on request.
2021/6/17 S. cerevisiae strains (BY29087-29102) and plasmids (BYP9784-9793) for characterization of reversible expression systems (The strains and plasmids contain yEVenus reporter of widely used inducible promoters and serve as a reference for future benchmarking of inducible systems) Gligorovski et al., (2020) have been deposited by Dr. S. J. Rahi (Swiss Federal Institute of Technology in Lausanne, Switzerland) and are now available on request.
2021/5/11 S. pombe mitochondrial-related strains(FY39903-39910, Uehara et al., (2021) ) deposited by Dr. M. Yanagida (OIST, Japan) are now available on request.
2021/05/10 S. japonicus strains isolated from Drosophila (FY39911-39918, Seike et al., (2021) ) deposited by Dr. T. Seike (Osaka Univ, Japan) are now available on request.
2021/3/2 S. pombe CRISPRi-related plasmids (FYP4849-4850, Ishikawa et al., (2021) ) deposited by Dr. K. Ishikawa (Kurume University, Japan) are now available on request.
2021/02/22 S. pombe meiosis rerated strains (FY39281-39583, Zhang et al. (2020)) deposited by Dr. T. Nakamura (Osaka City Univ, Japan) are now available on request.
2021/2/2 Genomic DNA of S. cerevisiae S288C, W303, X2180-1A and X2180-1B strains is now available on request (BYD1-4).
2021/1/29 A plasmid pUG6 derivative harboring S. cerevisiae URA3 (BYP9732) used in Sugiyama et al., (2008) have been deposited by Dr. S. Harashima (Sojo University, Japan) and are now available on request.
2021/1/29 Plasmids expressing S. cerevisiae ATG13 mutant series (BYP9771-9781) used in Kamada et al., (2010) and plasmids harboring 4xFLAG-kanMX and 4xFLAG-hphMX (BYP9782-9783) used in Sung et al., (2005) have been deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan) and are now available on request.
2021/1/24 S. cerevisiae strains including cell cycle and mating related mutants (BY10203-10447) have been deposited by Dr. S. Doi (Kinki University, Japan) and are now available on request.
2020/12/28 S. pombe strains (FY39764-39767, Nakazawa et al. (2019)) deposited by Dr. M. Yanagida (OIST, Japan) are now available on request.
2020/12/10 S. pombe tRNA-related strains (FY39278-39279) and plasmid (FYP4838, Porat and Bayfield. (2020) ) deposited by Dr. Mark Bayfield (York University) are now available on request.
2020/12/23 S. pombe actin cytoskeleton-related strains (FY39755, Takaine et al. (2014))(FY39756-39763, Morita et al. (2020)) and plasmids (FYP4839, Takaine et al. (2014) )(FYP4840-4848,Morita et al. (2020)) deposited by Dr. M. Takaine (Gunma University, Japan) are now available on request.
2020/12/2 S. pombe kinetochore related strains deposited by Dr. T. Toda (Hiroshima University) (Hsu and Toda., (2011) , FY39748-39754)are now available on request.
2020/8/18 S. pombe genome-wide lacO insertion library and strains deposited by Dr. D.Q. Ding (NICT) (Ding et al., (2019) , FY38751-39036) are now available on request.
2020/8/18 S. pombe pcp1-related strains deposited by Dr. T. Toda (Hiroshima University) (Tang et al., (2019) , FY39037-39038) are now available on request.
2020/4/7 S. cerevisiae plasmids related to YVH1 and GLN3 genes (BYP3302-3306) (BYP3383-3394) (BYP3413-3422) used in (Sakumoto et al., (2001) ) and (Numamoto et al., (2015) ) have been deposited by Dr. S. Harashima (Sojo University, Japan) and are now available on request.
2020/4/3 S. cerevisiae YIp plasmids for HSP30-GFP, HSP30-FLAG, ZRC1-GFP, MRH1-GFP, MRH1-mRFP, MRH1-FLAG, YRO2-GFP, YRO2-FLAG, GAT1-GFP, ADH6-GFP, ADH6-FLAG, ADH7-GFP, ADH7-FLAG, BDH1-GFP, BDH1-FLAG, CDC12-mCherry, BTN2-FLAG, BTN2-FLAG, SSA1-GFP, SSA4-GFP, HSP104-GFP, TSA1-GFP, YLL056c-GFP, YLL056c-FLAG and OLE1-GFP (BYP9706-9731) (Koma et al., (2012) ) (Izawa et al., (2010) ) (Takabatake et al., (2015) ) (Nakamura et al., (2017) ) (Nguyen et al., (2015) ) (Ishida et al., (2016) ) (Homoto and Izawa, (2018) ) (Yamauchi and Izawa, (2016) ) (Kato et al., (2018) ) (Kato et al., (2019) ) (Itooka et al., (2018) ) (Itooka et al., (2016) ) (Nguyen et al., (2018) ) have been deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) and are now available on request.
2020/4/2 S. pombe pof1+-related strains deposited by Dr. T. Toda (Hiroshima University) (Harrison et al., (2005) , FY38680-38683) are now available on request.
2020/3/3 S. pombe strains related to gamma tubulin deposited by Dr. T. Toda (Hiroshima University) are now available on request (FY38651-38656).
2020/1/30 PCR template plasmids pUG6-kanMX-CEN4 and pUG6-natMX-CEN4 (BYP9702-9703) for chromosome splitting in S. cerevisiae in addition to PCR template plasmids pSK+URA3-GAL1p and pSK+natMX-GAL1p (BYP9704-9705) for construction of conditional centromere by inserting GAL1p in region close to centromere were deposited by Dr. M. Sugiyama (Osaka University, Japan) and are now available on request.
2020/1/17 S. cerevisiae strain expressing Hsp104-yeGFP (BY29086) and plasmid pYES2/GFP (negative control plasmid for pYES2-α-synuclein-GFP) (BYP9686) (Takaine et al., (2019) ) were deposited by Dr. M. Takaine (Gunma University, Japan) and are now available on request.
2020/1/16 S. cerevisiae plasmids pYES2-α-synuclein-HA and pYES2-α-synuclein-GFP (Wijayanti et al., (2015) ) (BYP9684-9685) were deposited by Dr. H. Takagi (Nara Institute of Science and technology, Japan) and are now available on request.
2020/1/15 S. cerevisiae ydj1-1 mutant (Kimura et al., (1995) ) , cdc37-1 mutant (Kimura et al., (1997) ) and cdc48-3 mutants (Kimura et al., (2009) ) (BY29082-29085) were deposited by Dr. Y. Kimura (Shizuoka University, Japan) and are now available on request.
2020/1/10 A series of YIp-, YCp- and YEp-type vectors harboring a variety of drug-resistance genes as selection markers for S. cerevisiae (BYP9687-9701) were deposited by Dr. M. Sugiyama (Osaka University, Japan) and are now available on request.
2019/12/3 S. pombe strains related to TOR and MAP kinase (FY38598-38645) deposited by Dr. K. Shiozaki (Nara Institute of Science and Technology) are now available on request. (Shiozaki and Russell (1995) , Shiozaki and Russell (1996) , Shiozaki et ai., (1998) , Wang et ai., (2005) , Smith et ai., (2002) , Toda et ai., (1996) , Morigasaki et ai., (2019) )
2019/11/26 S. pombe strains(FY38586-38587) and plasmid(FYP4670) deposited by Dr. S. Martin (University of Lausanne, Switzerland) are now available on request.
2019/11/25 Improved two-hybrid vectors for S. cerevisiae (Tanaka, (2019) ) (BYP9680-9683) were deposited by Dr. S. Tanaka (Kochi University of Technology, Japan) and are now available on request.
2019/11/21 S. pombe strains deposited by Dr. T. Toda (Hiroshima University) (Takayama et al., (2010) , FY38588-38589), (Yukawa et al., (2019) , FY38590-38592) are now available on request.
2019/11/20 S. cerevisiae plasmids harboring several marker genes and plasmids used for chromosome splitting (BYP9661-9666) were deposited by Dr. Y. Sasano (Sojo University, Japan) and are now available on request.
2019/11/18 S. cerevisiae multi-copy genomic DNA libraries (YEp24(URA3) and YEp13(LEU2)) (Kamada et al., (2005) ) were deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan) and are now available on request.
2019/11/15 S. pombe tagging plasmids (FYP4236-4246) deposited by Dr. T. Toda (Hiroshima University) (Sato et al., (2005) ) are now available on request.
2019/11/7 S. pombe strains related to nuclear pore complex genes deposited by Dr. H. Asakawa (Osaka University) (Asakawa et al., (2014) , FY38419-38420,FY38435,FY38473,FY38477-38486), (Asakawa et al., (2019) , FY38421-38434,FY38436-38472,FY38474-38476) are now available on request.
2019/10/29 S. pombe strains (FY38487-38528) deposited by Dr. S. Martin (University of Lausanne, Switzerland) (Vjestica et al., (2019) ) are now available on request.
2019/10/21 S. pombe new series of stable integration vectors (SIV) (FYP4650-4703) deposited by Dr. S. Martin (University of Lausanne, Switzerland) (Vjestica et al., (2019) ) are now available on request.
2019/10/21 S. pombe microtubule-related strains deposited by Dr. T. Toda (Hiroshima University) (Toya et al., (2007) , FY34170-34171), (Yukawa et al., (2015) , FY34172), (Yukawa et al., (2018) , FY34173-34178), (Yukawa et al., (2019) , FY34179-34185), (Masuda et al., (2013) , FY38411-38418) are now available on request.
2019/10/11 S. pombe pheromone-related strains (FY34062-34125) and plasmids (FYP4596-4649, FYP4754-4759) deposited by Dr. T. Seike (RIKEN, Japan) (Seike et al., (2019) ) are now available on request.
2019/10/3 S. pombe strains deposited by Dr. M. Yanagida (OIST) (Nakazawa et al., (2018) , FY34127-34140), (Nakazawa et al., (2019a) , FY34141-34156), (Nakazawa et al., (2019b) , FY34157-34169) are now available on request.
2019/10/3 S. cerevisiae strains (BY29030-29032) and plasmids (BYP9649-9653) used in the unfolded protein response researches (Mori et al., (1996) , Mori et al., (1992) , Mori et al., (1993) ) were deposited by Dr. K. Mori (Kyoto University) and are now available on request.
2019/10/3 S. cerevisiae Sigam1278b-related strains and its mpr1 mpr2 disruptant (Takagi et al., (2000) , Roberts and Fink, (1994) , Shichiri et al., (2001) ) (BY29040-29042, BY29046) were deposited by Dr. G. R. Fink (Whitehead Institute/MIT) and Dr. H. Takagi (Nara Institute of Science and technology) and are now available on request.
2019/10/3 S. cerevisiae strains (BY29043-29045) used for isolating haploid clones that have same mating type and genotype as BY4741 or BY4742 through crossing with any haploid strains followed by random spore analysis and selection by auxotrophy were deposited by Dr. K. Kito (Meiji University) and are now available on request.
2019/10/3 S. cerevisiae strains (BY29047-29081) and plasmids (BYP9667-9679) used in RNA splicing and transcription related researches (Alexander et al., (2010) , Alexander et al., (2010) , Chathoth et al., (2014) , Barrass et al., (2015) , Aslanzadeh et al., (2018) , Mendoza-Ochoa et al., (2019) , Maudlin and Beggs, (2019) , Beggs, (1978) , Barrass and Beggs, (2019) , Swiatkowska et al., (2012) ) were deposited by Dr. Jean D. Beggs (University of Edinburgh) and are now available on request.
2019/9/28 S. pombe strains of the cytoskeleton-related gene deposited by Dr. I. Mabuchi (Gakushuin University) and Dr. J. Kashiwazaki (Kobe University)(Kashiwazaki et al., (2019) ), (FY30459-30652) are now available on request.
2019/6/24 Genomic DNA of S. cerevisiae S288C strain is now available on request (BYP9658)
2019/6/24 S. cerevisiae plasmids harboring loxP-natMX-loxP and loxP-AUR1-C-loxP cassettes were deposited and now available on request (BYP9659-9660)
2019/6/18 S. pombe strains expressing ATP-biosensor QUEEN-2m deposited by Dr. M. Takaine (Gunma University) and Dr. K. Kitamura (Hiroshima University) (Takaine et al., (2019) ), FY34060-34061) are now available on request.
2019/6/18 S. pombe strains related to the meiotic gene npg1 deposited by Dr. M. Takaine (Gunma University) (Takaine et al., (2014) ), (FY34046-34059) are now available on request.
2019/5/28 Saccharomyces cerevisiae strains and plasmids expressing ATP-biosensor QUEEN-2m that localizes in cytoplasm (+nucleus), mitochondria or on cytoplasmic surface of endoplasmic reticulum (Takaine et al., (2019) ) were deposited by Dr. Masakatsu Takaine (Gunma University) and are now available on request (BY29033-29038, BYP9654-9657)
2019/5/28 A Saccharomyces cerevisiae strain BY4743 was deposited by Dr. Yoshinobu Kaneko (Osaka University) and is now available on request (BY29039)
2019/4/1 S. pombe strains related to chromosome segregation and SAM deposited by Dr. M. Yanagida (OIST, Japan) (Hayashi et al. (2014)Hayashi et al. (2014)Takahashi et al. (2000)Nakamura et al. (2012)Hayashi et al. (2018)) are now available(FY33784-33861
2019/4/1 A Saccharomyces cerevisiae PercevalHR plasmid for monitoring intracellular ADP/ATP ratio (Nguyen et al., (2019) ) was deposited by Dr. Yukio Kimata (Nara Institute of Science and Technology) and is now available (BYP9646)
2019/4/1 Saccharomyces cerevisiae strains having ethanol production deficiency (Ida et al., (2012), Ida et al., (2013) ) were deposited by Dr. Hiroshi Shimizu (Osaka University) and are now available (BY29028-29029)
2019/4/1 Ogataea polymorpha mutant strains used for study for mating type switching and spindle pole body regulation (Yamamoto et al., (2017) , Maekawa et al., (2017)) were deposited by Dr. Hiromi Maekawa (Kyusyu University) and now are available (BY19092-19159)
2019/4/1 Saccharomyces cerevisiae strains harboring a variety of gene disruptions involved in HOG1/SLT2 pathways were deposited by Dr. Minetaka Sugiyama (Osaka University) and now are available (BY19160-19189)
2019/2/27 S. pombe plasmids for genome editing, CRISPR/Cas9, deposited by Dr. Aki Hayashi (Kwansei Gakuin University, Japan) (Hayashi and Tanaka. (2019)) are now available. (FYP4232-4235)
2019/1/16 S. pombe plasmids deposited by Dr. T. Nakagawa (Osaka University, Japan) (Okita et al. (2019))are now available. (FYP4222-4231)
2019/1/9 S. pombe strains deposited by Dr. T. Toda(Hiroshima University, Japan)(Vardy et al. (2000)Fong et al. (2010)Koyano et al. (2015)) are now available. (FY33772-33781)
2018/11/8 General purpose fission yeast vectors are now available (FYP4207-4217,FYP4221).
2018/10/26 S. pombe strains deposited by Dr. M. Kawamukai (Shimane University, Japan) (FY33673-33762), Dr. Y. Takayama (Teikyo University, Japan) (FY33763-33768), Dr. T. Toda (Hiroshima University, Japan)(FY33769-33771) and Dr. T. Nakamura (Osaka City University, Japan) (FY33526-33566) are now available.
2018/9/5 S. cerevisiae autophagy mutants (Takeshige et al., (1992)) (Tsukada and Ohsumi, (1993)) (Matsuura et al., (1997)) (BY18601-18926) and plasmids (BYP4895-4900) have been deposited by Dr. Y. Ohsumi, 2016 Nobel Prize laureate in Physiology or Medicine (Tokyo Institute of Technology, Japan) and are now available on request.
2018/9/5 S. cerevisiae sec mutants (BY18927-18951) have been deposited by Dr. A. Nakano (RIKEN, Japan) and are now available on request.
2018/6/19 S. pombe strains deposited by Dr. H. Masukata (Osaka University, Japan) are now available. (FY33573-33661)
2018/5/9 Metabolically engineered S. pombe strains using a CRISPR-Cas9 system (Ozaki et al., (2017)) deposited by Dr. T. Tanaka (Kobe University, Japan) are now available. (FY33185-33188)
2018/5/8 GFP-ATG8 (Suzuki et al., (2001)) (BYP9641-9642) and mCherry-ATG8 (Kamada et al., (2010)) (BYP9643-9645) deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan) are now available.
2018/5/8 Plasmids related to the PHO system in S. cerevisiae and O. polymorpha (Kaneko et al., (1987)) (BYP4865-4894) were deposited by Dr. Y. Kaneko (Osaka University, Japan) and are now available.
2018/5/8 S. cerevisiae mutants involved in the protein glycosylation (Wang et al., (1997)) (BY29020-29027) were deposited by Dr. Y. Shimma and Dr. T. Yoko-o (National Institute of Advanced Industrial Science and Technology, Japan) and are now available.
2017/11/20 Saccharomyces cerevisiae mutants for DNA replication constructed by using auxin-inducible degron (AID) and improved-AID (iAID) systems were deposited by Dr. H. Araki (National Institute of Genetics, Japan) (Miyazawa-Onami et al., (2017)). These strains are now available (BY28997-29019).
2017/10/20 Plasmids for visualization of RNA by Dr. Takeuchi-Andoh(Fission yeast (Takeuchi-Andoh et al., (2016))(FYP3987-3988), budding yeast(Takeuchi-Andoh et al., (2006))(BYP9639-9640) are now available.
2017/10/3 Fission yeast plasmids for estradiol-dependent induction deposited by Dr. Rhind (Ohira et al., (2017)) are now available(FYP3985-3986).
2017/9/15 About 1,800 fission yeast strains (FY31345-33184, including other yeast species) and 30 plasmids are now available.
2017/9/1 Budding yeast strains and plasmids used in researches for autophagy and TOR kinases were deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan). These strains (BY28944-28996) and plasmids (BYP9616-9638) are now available (Kamada et al., (2000), Kawamata et al., (2008), Kamada et al., (2010), Nakashima et al., (2008), Kamada, (2017), Kamada et al., (2005)).
2017/9/1 Ogataea polymorpha strains and plasmids used in genome editing by CRISPR/Cas were deposited by Dr. Y. Kaneko (Osaka University, Japan). These strains (BY28908-28943) and plasmids (BYP9596-9615) are now available (Numamoto et al., (2017)).
2017/5/15 Genome-wide segmental aneuploids of budding yeast were deposited by Dr. S. Harashima (Sojo University, Japan). These strains (BY28643-28692) are now available (Natesuntorn et al., (2015)).
2017/5/8 Multiple deletion strains of budding yeast protein phosphatase genes PTC1-PTC7 were deposited by Dr. S. Harashima (Sojo University, Japan). These strains (BY28520-28617) are now available (Sharmin et al., (2014)).
2017/5/8 Plasmids harboring loxP-ZeocinR-loxP, loxP-HygromycinR-loxP or loxP-CeruleninR-loxP were deposited by Dr. S. Harashima (Sojo University, Japan). These plasmids (BYP8999-9001) are now available (Sharmin et al., (2014)).
2017/4/28 Budding yeast mutants related to inositol biosynthesis were deposited by Dr. J. Nikawa (Kyushu Institute of Technology, Japan). These strains (BY19544-19567) are now available (Sugiyama et al., (2001)).
2017/4/28 gTOW plasmids (S. cerevisiae gene over-expression plasmids) were deposited by Dr. H. Moriya (Okayama University, Japan). These plasmids (BYP9021-9404) are now available (Makanae et al., (2013)).
2017/4/27 Fission yeast plasmids related to GFP-binding protein were deposited by Dr. Jin (Xiamen University). These plasmids (FYP3955-3984) are now available (Chen et al., 2017).
2016/12/06 Fission yeast resources related to exocytic pathway and sporulation (Strains: FY25555-25608, 25629-25667, 25758-25825, 28902-28954 and Plasmids: FYP3496-3504 etc) are now available (Imada and Nakamura, 2016).
2016/12/04 Fission yeast strains (FY29132-29607) and plasmids (FYP3792-3815) related to meiotic chromosome segregation, used in Yamamoto, A. and Hiraoka, Y. (2003), Hirose et al. (2011), Yoshida et al. (2013), Matsuhara and Yamamoto (2016) and Katsumata et al. (2016), are deposited by Dr. A. Yamamoto (Shizuoka Univ., Japan).
2016/12/04 Fission yeast strains (FY31339-31344) used in Nakazawa et al. (2015) deposited by Dr. M. Yanagida (OIST, Japan).
2016/10/17 Hybrid sake yeast strains constructed through a new crossbreeding approach were deposited by Dr. N. Fukuda (National Institute of Advanced Industrial Science and Technology (AIST), Japan). These strains (BY28505-28519) are available on request.
2016/10/11 Plasmids (BYP8941-8951) deposited by Dr. Y. Kaneko (Osaka University, Japan) are now available on request. These plasmids were used in a genetic study of PHO pathway in yeast Hansenula polymorpha.
2016/09/29 Wild fission yeast strains whose origin are different from Leupold L968 are deposited by Dr. J. Bahler (University College London, UK) . These strains (FY28955-29092) are available on request.
2016/09/28 Several cell cycle mutants of fission yeast are deposited by Dr. K. L. Gould (Vanderbilt University School of Medicine, USA). These strains (FY29093-29131) are available on request.
2016/06/14 The barcode yeast strains (BY27873-28253) deposited by Dr. S. Rutherford (Fred Hutchinson Cancer Research Center, USA) are now available on request. These strains were used in a study of yeast responses to osmotic stress.
2016/05/06 TOR-related strains (FY25826-25872) used in Ikai et al. (2011) deposited by Dr. M. Yanagida (OIST, Japan) is now available.
2016/03/14 90 gene knock out mutants are added to the fission yeast deletion mutant set deposited by Dr. K. Gould (Vanderbilt University, USA). They are not contained in the deletion mutant library set ver 3 (Bioneer corporation, Korea).
2016/03/09 A new fission yeast deletion set (90 strains) deposited by Dr. K. Gould (Vanderbilt University School of Medicine, USA) is now available for distribution(FY25668-25757).
2016/01/20 Thiamine-repressible expression vectors based on the backbone of the pREP-3X, pREP-41X and pREP-81X plasmids (Yeast. 2000;16(9):861-72.) deposited by Dr. C. Ribas (Univ. of Salamanca, Spain) are now available (18 plasmids, FYP3462-3479).
2016/01/07 Budding yeast strains (BY28357-BY28502) deposited by Dr. A. Nakano (RIKEN, Japan) have been available.
2015/12/11 Budding yeast strains (BY28260-BY28295) and plasmids (BYP8875-BYP8880) deposited by Dr. S. Tanaka (National Institute of Genetics, Japan) have been available.
2015/10/27 Fission yeast plasmids (FYP2932-2935) deposited by Dr. H. Nojima (Osaka Univ.) are now available.
2015/10/27 pTN575 (FYP2931), the vector of the cDNA library pTN-FC10, is now available.
2015/10/23 Two cDNA libraries, pTN-FC9 and pTN-FC10, are now available. These libraries contain cDNA, about 90% of which are full-length cDNA.
2015/10/09 Order of fission yeast strain sets are now available.
2015/10/09 We started to provide eMTA creation system.
2015/09/17 Kluyveromyces marxianus mutants (BY28297-BY28356; Yeast 30:485, 2013; Yeast 27:29, 2010) and plasmids (BYP8881-BYP8884; Yeast 31:29, 2014) were deposited by Dr. H. Hoshida (Yamaguchi University, Japan). They are now available on request.
2015/09/10 11cassettes for single-step drug-resistance and prototrophic marker switching in fission yeast (Yeast 2015) by Dr. A. Lorenz (Univ. of Aberdeen, UK) are now available (FYP2884-2894).
2015/07/15 35 'modules', including promoters, fluorescent tags and selection markers, to construct various plasmids in a one-step manner (Open Biol. 2015 Jun;5(6).) deposited by Dr. M. Sato (Waseda Univ.) are now available (FYP2895-2929).
2015/07/15 8 integrating vectors with mutated adh1 promotors deposited by Dr. Y. Watanabe (Univ. of Tokyo) are now available (FYP2874-2881).
2015/06/08 Fission yeast strains (FY17852-FY17875) deposited by Dr.T. Nakamura are now available.
2015/06/08 Fission yeast strains (FY25312-FY25336) deposited by Dr. M. Yanagida (OIST) are now available.
2015/5/22 Fission yeast strains (FY23587-FY23592, FY23594, FY23596, FY23601-FY23604, FY23606-FY23610, FY23616-FY23619, FY23625, FY23626, FY23676-FY23680) deposited by Dr. T. Sugiyama (NIH) are now available.
2015/05/12 Non-standard S. pombe strains are now available. (FY25283-FY25301, FY25303-FY25311)
2015/04/28 Fission yeast strains (FY24008-25242, FY25257-FY25275) deposited by Dr. Y. Watanabe (University of Tokyo) are now available.
2015/04/28 Fission yeast strains (FY25243-25248) deposited by Dr. H.Murakami (Chuo University) are now available.
2015/04/28 Fission yeast strains deposited by Dr. J. Mata (Univ. Cambridge, UK) are now available for distribution(FY25251-FY25256).
2014/09/16 A new fission yeast deletion set deposited by Dr.K. Gould (Vanderbilt University School of Medicine, USA) is now available for distribution (FY23693-23986).
2014/09/16 Fission yeast strains deposited by Dr. R. Weismann (Open University of Israel, Israel) are now available for distribution(FY23988-24006).
2014/08/28 pFA6a plasmids carrying drug-resistant markers, (Nat, Ble or Hyg) deposited by Dr. A.W. Carr (University of Sussex, UK) are now available (FYP2869-2871).
2014/06/11 Budding yeast vectors (pRS42x; Gene 110:119, 1992) deposited by Dr. H. Moriya (Okayama University, Japan) are now available. The vectors are BYP7838 to BYP7841.
2014/05/09 Fission yeast mutants (structural genes for pheromone) are now available (FY22789-22972).
2014/04/14 Budding yeast resources related to ribosome biogenesis (deposited by Dr. K. Mizuta, Hiroshima University, Japan) are now available. Strains are BY26955 to BY26963 and plasmids are BYP7851 to BYP7871.
2013/10/15 Fission yeast ts and cs mutant sets are now available (FY22064-22708). These mutants are deposited by Dr. T. Tani (Kumamoto University).
2013/10/15 Fission yeast mug gene disruptant set is deposited by Dr. S. Moreno (Salamanca University).
2013/10/15 Fission yeast pheromone-related gene disruptants are now available.
2013/10/11 Budding yeast mutants deposited by Dr. H. Hoshida (Yamaguchi University, Japan) are now available. (BY25957-BY26000, BY26944-BY26954; Yeast 30:243, 2013)
2013/07/24 Budding yeast expression vectors (BYP7579-BYP7588) deposited by Dr. J. Ishii (Kobe Univ., Japan) and gTOW vectors (BYP7589, BYP7590) deposited by Dr. H. Moriya (Okayama Univ., Japan) are now available.
2013/06/12 Fission yeast strains (FY21635-21664) deposited by Dr. H. Tohda (Asahi Glass Co. Ltd, Japan) are now available.
2013/05/29 Fission yeast strains (FY21828-21832) deposited by Dr. K. Nakano (Tsukuba University) are now available.
2013/03/18 Auxin-inducible-degron plasmids (BYP7425 - BYP7435; Mol. Cell, 2013 http://dx.doi.org/10.1016/j.molcel.2013.02.012) deposited by Dr. M. Kanemaki (National Institute of Genetics, Japan) are now available in the budding yeast resources.
2013/02/25 Budding yeast mutants related to ribosome biogenesis (BY25930-BY25946; deposited by Dr. K. Mizuta, Hiroshima University, Japan) and drug hypersensitive pdr1 pdr3 double mutant (BY25929; deposited by Dr. M. Ueki, RIKEN, Japan) are now available.
2012/12/10 A series of PGK1-promoter-expression vectors for the budding yeast (BYP7357 - BYP7373) was deposited by Dr. J. Ishii (Kobe University, Japan) and now available.
2012/08/09 Budding yeast plasmids for stress granules markers (BYP7348 - BYP7356) were deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) and now available.
2012/05/25 Budding yeast plasmids related to ribosome biogenesis (BYP7304 - BYP7324) were deposited by Dr. K. Mizuta (Hiroshima University, Japan) and now available.
2012/03/16 Budding yeast auxin-inducible degron strains (BY25917 to BY25927) deposited by Dr. M. Kanemaki (National Institute of Genetics, Japan) and GFP-fusion plasmids for visualization of P-body in the budding yeast (BYP7215 and BYP7216) deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) are now available.
2012/01/31 Fission yeast strains (FY21444-21460) deposited by Dr. S. Sazer (Baylor College of Medicine) are now available.
2012/01/31 Fission yeast strains (FY21462-21477) deposited by Dr. S. Hauf (Friedrich Miescher Laboratory of the Max Planck Society) are now available.
2011/12/12 Heat-inducible degron system for S. pombe (Plasmids, FYP2779-2786)deposited by Prof. Stephen Kearsey (Oxford University) is now available.
2011/11/25 Auxin-inducible degron system for S. pombe (Strains; FY21102,21103,21104, and Plasmid, FYP2827) deposited by Prof. Masukata (Osaka University) is now available.
2011/05/10 Septin-related budding yeast strains (BY25624 to BY25887) deposited by Dr. M. Iwase (University of Tokyo, Japan) are now available.
2011/02/25 Budding yeast septin-related plasmids (BYP6854 to BYP7144) deposited by Dr. M. Iwase (University of Tokyo, Japan) are now available.
2011/02/23 Fission yeast strains (24 strains; FY19908-FY19931) deposited by Dr. C. Shimoda (Osaka City University, Japan) are now available.
2011/02/23 Fission yeast strains (23 strains; FY19781-FY19806) deposited by Drs. H. Nojima and D. Okuzaki (Osaka University, Japan) are now available.
2011/02/23 Fission yeast strains (284 strains; FY19055-FY19373) deposited by Dr. J. Bahler (University College London, London, UK) are now available.
2011/02/08 Ribosome biosynthesis-related mutants (BY25900 to BY25915) was deposited by Prof. K. Mizuta (Hiroshima University, Japan) and are available now for distribution.
2011/01/14 Now sequences of full length cDNAs are available.
2010/12/28 Budding yeast strains expressing tagged proteasome subunit (BY25889 to BY25899) were deposited by Dr. Y. Saeki (Tokyo Metropolitan Institute of Medical Science, Japan) and are now available.
2010/12/17 From April 2009 onwards, the Genome Viewer was displaying incorrect information about S. pombe genomic DNA clones. We apologize for this error and would like to offer a retraction.Specifically, DNA clones mapped to after 3.7Mbp of chromosome 1 were wrongly displayed by a maximum of 6150bp. This was caused by the usage of an older version of genome sequence to analyze the homologous region of DNA clones.As a result, if you are using clones mapped to after 3.7Mbp of chromosome 1,there is a possibility that the desired gene is not included in the clone.The resource center is going to contact and resend the correct clones for all previous orders but if you are in a hurry, please contact us.We will strive to avoid such mistakes in the future and we hope to receive your continuous support. Yukiko Yamazaki of NBRP Information Center
2010/12/01 Double promoter (TEF1 and PGK1)-vector plasmids (BYP7150 and BYP7151; Yeast 27:955, 2010) was deposited by Prof. Jens Nielsen (Chalmers University of Technology, Sweden) and are now available.
2010/11/11 Fission yeast strains (337 strains) deposited by Dr. J. Kohli (Bern University, Switzerland) are now available.
2010/11/11 S. pombe plasmids for gene-tagging/-fusion deposited by Dr. S. Forsburg (Univ. Southern California) are now available.
2010/09/02 Auxin-inducible-degron related plasmids (BYP6851 - BYP6853; Dr.M. Kanemaki, Osaka Univ., Japan), Fluorescent protein-expressed strains (BY25610 BY25612; M. Carmi, Weizmann Inst. of Science , Israel) and rad mutants (BY25614 BY25623; YGRC) are available now for distribution.
2009/12/01 Dr.M. Kanemaki (Osaka Univ., Japan) has deposited the auxin-inducible degron system and its related strains (BYP6739 BYP6744, BY255576 BY25603), which were published in Nature Methods online (doi: 10.1038/nmeth.1401).
2009/10/01 Akada's budding yeast genomic library clones (BYP6618-BYP6738) and budding yeasts (BY25550-BY25557) from R. Akada (Yamaguchi Univ) are available for disitribution.
2009/08/19 Budding yeast plasmids (BYP6604 - BYP6617) from Dr.A. Sakai (MITILS) are available for distribution.
2009/05/20 Fission yeast strains (FY17884-FY17930) from Dr.T. Tani (Kumamoto Univ) and from Teresa Wang (Stanford Univ) were added to our collection.
2009/04/17 Akada's budding yeast genomic library clones (BYP6475-BYP6538) and OUT budding yeasts (BY19901-BY19992) are available for disitribution.
2009/03/17 Ninety-eitht fission yeast full-length cDNA clones are added to our collection (FYC1484-FYC1581).
2009/03/06 Dr.H. Takagi (NAIST, Japan) has deposited the budding yeast mutants and plasmids (Genes Cells 13:105, 2008). BYP6472-BYP6474: HA-tagged HSF1, MSN2, and MSN4. BY25535-BY25541: rsp5 mutant and related strains.
2009/01/23 Dr.Akada’s library clones carrying budding yeast genes (BYP6413-BYP6469; 57 plasmids) and double disruptants of protein phosphatase gene (SIW14 or PPZ1) and protein kinase gene (BY10001-BY10202) are available for distribution.
2008/12/25 Fission yeast strains (FY17835-FY17851) from Dr.H. Nojima (Osaka) and from Dr.N. Hamasaki-Katagiri (Maryland) were added to our collection.
2008/11/25 A set of GFP-fusion strains (1,060 strains; FY14458-FY15517) of fission yeast are now available for distribution. These strains were constructed by Yasushi Hiraoka's lab (Osaka Univ./NICT).
2008/11/18 Fission yeast strains (FY17729-17834) are deposited by Dr.Yasushi Hiraoka (Osaka Univ. / KARC), Hiroshi Murakami (Nagoya City Univ.) and Dr.Makoto Kawamukai (Shimane Univ.).
2008/10/07 Akada’s library clones carrying budding yeast genes (BYP6337 - BYP6412; 76 plasmids) are available for distribution.
2008/09/03 Fission yeast strains (FY17641-17728, 84 strains) are deposited by Dr.Takashi Toda(Cancer Research UK). These strains have been reported in recent issues of Nature (PMID17476213) and Nature Cell Biol (PMID17486116).
2008/08/26 Fission yeast GFP-tagging strains constructed by Yasushi Hiraoka's Lab are added to our resource collection.
2008/08/12 New budding yeast resources, i.e., [BYP6238-BYP6254, BYP6256-BYP6318; pAK plasmids] from A. Toh-e (Rinshoken), [BYP6327-BYP6334; J. Biol. Chem. 280:35822, 2005] from Dr.Y. Kikuchi (Univ. of Tokyo) & Dr.Y. Takahashi (NIH, USA), and [BYP6335 and BYP6336, plasmids for split-GFP method; Fungal Genet. Biol. 45:597-604 (2008)] from D. J. Timson (Queen's Univ.), are available for distribution.
2008/06/17 S. pombe full-length cDNA clones are added (Release-3; 42 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data.
2008/06/04 S. pombe genomic DNA clones are now available for distribution. Please click "Physical Maps" on this page, then you may find genomic clones mapped on chromosomes. Random Sau3A fragments from a wild-type S. pombe strain L972 were cloned into the pAL-KS vector. Order by E-mail (nbrpombe@sci.osaka-cu.ac.jp) is welcome.
2008/05/30 New fission yeast strains [FY17627-17637] from M. Yanagida and R. Sugiura (kinki Univ.) are available for distribution. S. pombe plasmids (8 clones) from Taro Nakamura (Osaka City Univ.) are added to the collection.
2008/05/29 New fission yeast strains [FY17237-17355]from M. Yanagida (50 strains; Kyoto Univ.), T. Tani (8 strains; Kumamoto Univ.) and T. Nakamura (50 strains; Osaka City Univ.) are available for distribution.
2008/03/06 S. pombe full-length cDNA clones are added (Release-2, 482 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data.
2008/02/25 New fission yeast strains [FY17233-FY17236] from M. Ueno (Hiroshima Univ.) are available for distribution.
2008/02/08 New budding yeast resources, [BYP6237, expression plasmid] from R. Akada (Yamaguchi Univ.) and [BYP5346-BYP5584, TOp plasmids (first batch)] from A. Toh-e (Rinshoken), are available for distribution.
2008/01/09 New budding yeast resources, [BYP6221-BYP6236, expression plasmids] from R. Akada (Yamaguchi Univ.) and [BY25525-BY25530, aryl hydrocarbon-reporter yeasts] from M. Kawanishi (Osaka Prefecture University), are available for distribution.
2007/12/26 New fission yeast strains [FY17223-17232] and plasmids [FYP2224-2229] from Kentaro Nakano (Tsukuba Univ.) and from Takayoshi Kuno (Koe Univ.), respectively, are available for distribution.
2007/12/07 New budding yeast resources, [BY25470-BY25473, ste mutants] from R. Akada (Yamaguchi Univ.) and [BY25520-BY25524, SUMO related mutants] from Y. Takahashi & Y. Kikuchi (NIH, USA & Univ. of Tokyo), are available for distribution.
2007/11/12 S. pombe full-length cDNA clones are now available (Release-1, 958 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data. Release-2 will come in a few weeks.
2007/11/06 New budding yeast resources (BY25450-BY25469, Sake yeast derivatives) from R. Akada (Yamaguchi Univ.) are available for distribution.
2007/10/24 New fission yeast plasmids are deposited by Y. Hama (Asahi Glass Co.), M. Yanagida (Kyoto Univ.) and A. Ishihama (Hosei Univ.). They are available for distribution [FYP2027-2217].
2007/10/19 New budding yeast resources [BY21796-BY21799 (K. lactis), BY25348-BY25449, BYP6160-BYP6204, BYP6215-BYP6216, BYP6217-BYP6220 (plasmids for K. lactis)] are available for distribution.
2007/10/11 S. pombe strains carrying six new amino acid-auxotrophic markers deposited by T. Kuno are available for distribution (Curr Genet 52: 97-105, 2007). Suitable for gene disruption.
2007/08/03 NBRP-Yeast is linked to GeneDB (S. pombe) and SGD (S. cerevisiae) through the genetic symbols marked with underline at the result page of strain retrieval.
2007/06/13 Large-scale-deletion budding yeast strains (BYP21770 - BYP21787; Appl. Microbiol. Biotechnol. 75:589, 2007) are available for distribution, except for BY21770, BY21771 and BY21779.
2007/05/08 Akada DNA library (BYP6127 - BYP6145; Mol. Gen. Genet. 254:267, 1997) is now available for distribution in the budding yeast plasmid resources.
2007/04/16 We renewed this site.
2007/04/11 Protease-deficient S. pombe mutants deposited by Dr. Y. Hama (ASPEX Division,Asahi Glass Co. Ltd.) are now available for distribution (FY16976-16979).