 |
|
|
|
|
||
|
|
Notice:Resumption of distribution of budding yeast resources
Hi, everyone!! Long time no see! How have you been?
We have resumed distribution of budding yeast resources from June 1st. We are looking forward to receiving your order!
We have resumed distribution of budding yeast resources from June 1st. We are looking forward to receiving your order!
National BioResource Project - Yeast
In order to continue YGRC activity, your support and help are very important.
When you publish the outcome by using the NBRP yeast resources, please refer YGRC as the source of the yeast resource in your publication. For example, " (Resource name) was provided by the NBRP, Japan." In addition, please visit the "Research Paper Online Registration Site" and give us the related information after publication. Your cooperation will be highly appreciated.
A protocol for S. cerevisiae genome editing with a series of simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vectors(BYP9733-9770, BY9805-9817) （Okada et al., 2021) was provided by Dr. S. Okada (Kyusyu University, Japan).
S. cerevisiae Genome-editing protocol
What's New
Display of all
Display of all
| 2021/9/24 | S. pombe strains (FY39933-39966) and plasmids (FYP4966-5019) for iRFP and phytochrome-based optogenetics deposited by Dr. Y. Goto (National Institute for Basic Biology, Japan, Sakai et al. (2021)) are now available on request. |
| 2021/9/15 | S. pombe auxin-inducible degron system plasmids (FYP4964-4965) and strains (FY39929-39932) deposited by Dr. T. Sugiyama (Shanghai Tech University, China, Song et al. (2021)) are now available on request. |
| 2021/9/13 | A series of simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vectors for S. cerevisiae genome editing (BYP9733-9770) (BYP9805-9817) (Okada et al., (2021) ) have been deposited by Dr. S. Okada (Kyusyu University, Japan) and are now available on request. A protocol for S. cerevisiae genome editing with these plasmids was also provided by Dr. S. Okada and posted on the top page. |
| 2020/8/27 | S. pombe auxin-inducible degron system strains (FY39919-39928) and plasmids (FYP4960-4963) deposited by Dr. Li-Lin Du (NIBS, China, Zhang et al. (2021)) are now available on request. |
| 2021/7/5 | A series of simple to use CRISPR/Cas vectors using SpCas9, SaCas9 and AsCas12a with distinct PAMs for genome editing (BYP9733-9770) used in Okada et al., (2021) have been deposited by Dr. S. Okada (Kyusyu University, Japan) and are now available on request. |
Resources
Service fee will be charged for each bioresource provided
510 JPY system maintenance fee per order, with no limitation for numbers of resource provided, will also be charged to secure our valued customers' information and protect them from credit card fraud. Shipping fee will be separately added as it costs.
Payments only with major credit cards (visa, master) will be accepted.
Please e-mail to; contactus-yeast@shigen.info, for any questions or concerns you may have.
Payments only with major credit cards (visa, master) will be accepted.
Please e-mail to; contactus-yeast@shigen.info, for any questions or concerns you may have.
Service fee will be charged for each bioresource provided;
| Academia | Private Organization | |
| Yeast stock, Plasmid | 510 JPY | 11,300 JPY |
| Full length cDNA, Genome DNA clone | 620 JPY | 17,400 JPY |
| DNA library | 10,200 JPY - | 71,300 JPY - |
| Set order | 30,600 JPY - | 110,000 JPY - |
Shipping fee
| Japan | Other country | |
| Postage | 120 JPY | 400 JPY |
| Yamato transport | COD | - |
| EMS | - | 1,400 JPY |
| FedEx | - | COD |
*When you have chosen FedEx delivery, the envelope is basically packed into "FedEx Clinical Pak," which is 310 x 240 mm.
Electron microscope images of S. cerevisiae and S. pombe were provided by Dr. M. Osumi, Integrated Imaging Research Support.
Fluorescence microscope image of S. cerevisiae was provided by Dr. Y. Ohya, University of Tokyo.
Fluorescence microscope image of S. pombe was provided by Dr. C. Shimoda, Osaka City University.
Fluorescence microscope image of S. cerevisiae was provided by Dr. Y. Ohya, University of Tokyo.
Fluorescence microscope image of S. pombe was provided by Dr. C. Shimoda, Osaka City University.