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National BioResource Project - Yeast
In order to continue YGRC activity, your support and help are very important.
When you publish the outcome by using the NBRP yeast resources, please refer YGRC as the source of the yeast resource in your publication. For example, " (Resource name) was provided by the NBRP, Japan." In addition, please visit the "Research Paper Online Registration Site" and give us the related information after publication. Your cooperation will be highly appreciated.
A protocol for S. cerevisiae genome editing with a series of simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vectors(BYP9733-9770, BY9805-9817) （Okada et al., 2021) was provided by Dr. S. Okada (Kyusyu University, Japan).
S. cerevisiae Genome-editing protocol
What's New
Display of all
Display of all
| 2023/05/09 | S. cerevisiae BY4741 Δadh1 strain (BY29295) ( Utsumi et al. (2023) ) that is not included in the commercially available yeast KO strain collection has been deposited by Dr. N. Miura (Osaka Metropolitan University, Japan) and is now available on request. |
| 2023/05/08 | S. pombe chromosome-related strains deposited by Dr. Y. Chikashige (NICT, Japan) ((FY41101-41248, FY41327, Chikashige et al., 2004, 2006, 2007, 2009, 2014, 2017) (FY41249-41301, Yamamoto et al. (2019)) are now available on request. |
| 2023/4/17 | S. cerevisiae YEp plasmids (BYP9966-9967) for the expression of mitochondria-targeted GFP, that was used in Okamoto et al. (2016) and Ishikawa et al. (2022) , have been deposited by Dr. I. Miyakawa (Yamaguchi University, Japan) and Dr. S. Izawa (Kyoto Institute of Technology, Japan), and are now available on request. |
| 2023/4/12 | S. cerevisiae 25 YIp plasmids (BYP9941-9965) for the expression of GFP-, mRFP- or FLAG-tagged Rpn1, Rpn7, Pre6, Hsp60, Hsp78, Ssc1, Ilv2, Kgd1, Aco1, Fes1, Sis1, Ssa1, Ssa3 and Lsg1, that were used in Nguyet et al. (2022) , Ishikawa et al. (2022) and Yoshida et al. (2021) , have been deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) and are now available on request. |
| 2023/03/22 | S. pombe tubulin-related plasmids ( FYP5207-FYP5208) used in Murase et al., (2022) deposited by Dr. Sato (Waseda University, Japan) are now available on request. |
Resources
Service fee will be charged for each bioresource provided
510 JPY system maintenance fee per order, with no limitation for numbers of resource provided, will also be charged to secure our valued customers' information and protect them from credit card fraud. Shipping fee will be separately added as it costs.
Payments only with major credit cards (visa, master) will be accepted.
Please e-mail to; contactus-yeast
shigen.info, for any questions or concerns you may have.
Payments only with major credit cards (visa, master) will be accepted.
Please e-mail to; contactus-yeast
shigen.info, for any questions or concerns you may have.
Service fee will be charged for each bioresource provided;
| Academia | Private Organization | |
| Yeast stock, Plasmid | 510 JPY | 11,300 JPY |
| Full length cDNA, Genome DNA clone | 620 JPY | 17,400 JPY |
| DNA library | 10,200 JPY - | 71,300 JPY - |
| Set order | 30,600 JPY - | 110,000 JPY - |
Shipping fee
| Japan | Other country | |
| Postage | 120 JPY | 400 JPY |
| Yamato transport | COD | - |
| EMS | - | 1,900 JPY |
| FedEx | - | COD |
*When you have chosen FedEx delivery, the envelope is basically packed into "FedEx Clinical Pak," which is 310 x 240 mm.
Electron microscope images of S. cerevisiae and S. pombe were provided by Dr. M. Osumi, Integrated Imaging Research Support.
Fluorescence microscope image of S. cerevisiae was provided by Dr. Y. Ohya, University of Tokyo.
Fluorescence microscope image of S. pombe was provided by Dr. C. Shimoda, Osaka Metropolitan University.
Fluorescence microscope image of S. cerevisiae was provided by Dr. Y. Ohya, University of Tokyo.
Fluorescence microscope image of S. pombe was provided by Dr. C. Shimoda, Osaka Metropolitan University.