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Budding yeast (plasmids)  BYP9678
Academia:510JPY/plasmid , Private Organization:11,300JPY/plasmid
NBRP IDBYP9678 
Lab No
Plasmid Namep4Fui- ΔPEST
Host nameDH5α
Inserted geneThe PEST motif of S. cerevisiae Fui1 has been deactivated, so the permease is not degraded when there is sufficient intracellular uracil for the cell’s needs. Works well in 4tU labelling experiments and improves pulse/chase performance. pRS426 derivative
Vector TypeYEp
Selection Marker
(E. coli)
Amp
Selection Marker
(S. cerevisiae)
URA3
Source
(depositor)
Jean D. Beggs:Wellcome Trust Centre for Cell Biology, University of Edinburgh
[J.Beggs@ed.ac.uk]
Journal Please submit your publication
Ref
J. Vis. Exp. 150 (2019) doi: 10.3791/59952.
[https://www.ncbi.nlm.nih.gov/pubmed/?term=Extremely+rapid+and+specific+metabolic+labelling+of+RNA+in+vivo+with+4-thiouracil]
TermsA
CommentThe PEST motif of S. cerevisiae Fui1 has been deactivated, so the permease is not degraded when there is sufficient intracellular uracil for the cell’s needs. Works well in 4tU labelling experiments and improves pulse/chase performance. pRS426 derivative. Important information for user, (YGRC attachment)
LMOP1