| NBRP ID | BYP9678  |
| Lab No | |
| Plasmid Name | p4Fui- ΔPEST |
| Host name | DH5α |
| Inserted gene | The PEST motif of S. cerevisiae Fui1 has been deactivated, so the permease is not degraded when there is sufficient intracellular uracil for the cell’s needs.
Works well in 4tU labelling experiments and improves pulse/chase performance. pRS426 derivative |
| Vector Type | YEp |
Selection Marker
(E. coli) | Amp |
Selection Marker
(S. cerevisiae) | URA3 |
Source
(寄託者) | |
| 成果論文 | 
|
| Ref | |
| Terms | A |
| Comment | The PEST motif of S. cerevisiae Fui1 has been deactivated, so the permease is not degraded when there is sufficient intracellular uracil for the cell’s needs.
Works well in 4tU labelling experiments and improves pulse/chase performance. pRS426 derivative. Important information for user, (YGRC attachment) |
| LMO | P1 |
