NBRP::Yeast

NBRP ID	Lab No	Plasmid Name	Host name	Inserted gene	Vector Type	Selection Marker (E. coli)	Selection Marker (S. cerevisiae)	Comment	Source (depositor)	Terms*2	LMO
BYP9733	12-67	YIplac128-pGAL1-Cas9-tADH1	DH5α	pGAL1-Cas9-tADH1	YIp	Amp	LEU2	For SpCas9 expression	Okada, Satoshi	B-1	P1
BYP9734	15-48	YIpHIS-pGAL1-Cas9-tADH1	DH5α	pGAL1-Cas9-tADH1	YIp	Amp	HIS3	For SpCas9 expression	Okada, Satoshi	B-1	P1
BYP9735	9-81	YIplac128-pGAL1-yxCas9(3.7)-tADH1	DH5α	pGAL1-yxCas9(3.7)-tADH1	YIp	Amp	LEU2	For xCas9(3.7) expression, xCas9(3.7) codon usage is optimized for S. cerevisiae	Okada, Satoshi	B-1	P1
BYP9736	15-58	YIplac128-pGAL1-ySaCas9-tADH1	DH5α	pGAL1-ySaCas9-tADH1	YIp	Amp	LEU2	For SaCas9 expression, SaCas9 codon usage is optimized for S. cerevisiae	Okada, Satoshi	B-1	P1
BYP9737	13-14	YIplac128-pGAL1-yenAsCas12a-tADH1	DH5α	pGAL1-yenAsCas12a-tADH1	YIp	Amp	LEU2	For enAsCas12a expression, enAsCas12a codon usage is optimized for S. cerevisiae	Okada, Satoshi	B-1	P1
BYP9738	8-17	pSNR52-2BsaI-sgRNAFE(empty)-tSUP4-CU	DH5α	pSNR52-2BsaI-sgRNAFE(empty)-tSUP4	YCp	Amp	URA3	Golden Gate Assembly destination vector for SpCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9739	12-50	pSNR52-2BsaI-sgRNAFE(empty)-tSUP4-CH	DH5α	pSNR52-2BsaI-sgRNAFE(empty)-tSUP4	YCp	Amp	HIS3	Golden Gate Assembly destination vector for SpCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9740	9-33	pGAL1-2BsaI-sgRNAFE(empty)-HDV-tADH1-CU	DH5α	pGAL1-2BsaI-sgRNAFE(empty)-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for SpCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9741	12-80	pGAL1-tRNA-2BsaI-sgRNAFE(empty)-HDV-tADH1-CU	DH5α	pGAL1-tRNA-2BsaI-sgRNAFE(empty)-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for SpCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9742	9-57	pSNR52-2BsaI-SagRNA(empty)-tSUP4-CU	DH5α	pSNR52-2BsaI-SagRNA(empty)-tSUP4	YCp	Amp	URA3	Golden Gate Assembly destination vector for SaCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9743	9-58	pGAL1-2BsaI-SagRNA(empty)-HDV-tADH1-CU	DH5α	pGAL1-2BsaI-SagRNA(empty)-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for SaCas9 sgRNA expression	Okada, Satoshi	B-1	P1
BYP9744	13-52	pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for enAsCas12a crRNA expression	Okada, Satoshi	B-1	P1
BYP9745	15-13	pGAL1-Cas9-tADH1-pSNR52-2BsaI-sgRNAFE(empty)-tSUP4-CU	DH5α	pGAL1-Cas9-tADH1-pSNR52-2BsaI-sgRNAFE(empty)-tSUP4	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with SpCas9	Okada, Satoshi	B-1	P1
BYP9746	15-12	pGAL1-Cas9-tADH1-pGAL1-2BsaI-sgRNAFE(empty)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-2BsaI-sgRNAFE(empty)-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with SpCas9	Okada, Satoshi	B-1	P1
BYP9747	16-15	pGAL1-Cas9-tADH1-pGAL1-2BsaI-sgRNAFE(empty)-HDV-tCYC1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-2BsaI-sgRNAFE(empty)-HDV-tCYC1	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with SpCas9	Okada, Satoshi	B-1	P1
BYP9748	17-31	pGAL1-ySaCas9-tADH1-pGAL1-2BsaI-SagRNA(empty)-HDV-tCYC1-CU	DH5α	pGAL1-ySaCas9-tADH1-pGAL1-2BsaI-SagRNA(empty)-HDV-tCYC1	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with SaCas9	Okada, Satoshi	B-1	P1
BYP9749	15-23	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tADH1	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9750	16-16	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tCYC1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(empty)-2BsaI-U4AU4-HDV-tCYC1	YCp	Amp	URA3	Golden Gate Assembly destination vector for genome editing with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9751	15-59	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-1)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-1)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9752	15-60	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-2)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-2)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9753	15-62	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-4)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-4)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9754	15-63	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-5)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-5)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9755	15-64	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-6)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-6)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9756	15-66	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-8)-HDV-tADH1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-HH-sgRNAFE(CSE4-8)-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with SpCas9	Okada, Satoshi	B-1	P1
BYP9757	16-17	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-1)-tSUP4-CU	DH5α	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-1)-tSUP4	YCp	Amp	URA3	For genome editing at CDC3 with SpCas9	Okada, Satoshi	B-1	P1
BYP9758	16-18	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-2)-tSUP4-CU	DH5α	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-2)-tSUP4	YCp	Amp	URA3	For genome editing at CDC3 with SpCas9	Okada, Satoshi	B-1	P1
BYP9759	16-19	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-3)-tSUP4-CU	DH5α	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-3)-tSUP4	YCp	Amp	URA3	For genome editing at CDC3 with SpCas9	Okada, Satoshi	B-1	P1
BYP9760	16-20	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-4)-tSUP4-CU	DH5α	pGAL1-Cas9-tADH1-pSNR52-sgRNAFE(CDC3-4)-tSUP4	YCp	Amp	URA3	For genome editing at CDC3 with SpCas9	Okada, Satoshi	B-1	P1
BYP9761	16-44	pGAL1-Cas9-tADH1-pGAL1-sgRNAFE(ORC6N1)-HDV-tCYC1-CU	DH5α	pGAL1-Cas9-tADH1-pGAL1-sgRNAFE(ORC6N1)-HDV-tCYC1	YCp	Amp	URA3	For genome editing at ORC6 with SpCas9	Okada, Satoshi	B-1	P1
BYP9762	15-69	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-3)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-3)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9763	15-70	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-4)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-4)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9764	15-71	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-5)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-5)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9765	15-72	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-6)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-6)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9766	15-73	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-7)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-7)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9767	15-74	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-8)-U4AU4-HDV-tADH1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(CSE4-12a-8)-U4AU4-HDV-tADH1	YCp	Amp	URA3	For genome editing at CSE4 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9768	16-45	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(ORC6T1)-U4AU4-HDV-tCYC1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AsCpf1gRNA(ORC6T1)-U4AU4-HDV-tCYC1	YCp	Amp	URA3	For genome editing at ORC6 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9769	16-46	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(ORC6T2)-U4AU4-HDV-tCYC1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AsCpf1gRNA(ORC6T2)-U4AU4-HDV-tCYC1	YCp	Amp	URA3	For genome editing at ORC6 with enAsCas12a	Okada, Satoshi	B-1	P1
BYP9770	16-70	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AscrRNA(SMT3T1)-U4AU4-HDV-tCYC1-CU	DH5α	pGAL1-yenAsCas12a-tADH1-pGAL1-tRNA-AsCpf1gRNA(SMT3T1)-U4AU4-HDV-tCYC1	YCp	Amp	URA3	For genome editing at SMT3 with enAsCas12a	Okada, Satoshi	B-1	P1